Virgin Little Sister

[Kokonoki Nao] Virgin [Digital]

(COMIC1☆14) [squeezecandyheaven (Ichihaya)] Imouto wa Genius + Omake | My Little Sister Is a Genius + Bonus Story [English] [sneikkimies]

(Mimiket 5) [Imomuya Honpo (Azuma Yuki)] Oniisama e... 2.6 Mimiket 5 Kinen Copy Bon (Sister Princess)

[Miharu] Imouto no Betatsuku Hada to Sono Kusai | My Little Sister's Sticky Body and Odor (COMIC Masyo 2008-12) [English] [desudesu]

[Magukappu (Magukappu)] DIACO (Little Witch Academia)

[Virgin Virus2 (Matsumi Jun)] O jou no Tsuyosugiru Seiyoku

(C84) [Tennenseki (Suzuri)] SUN SUN Kud (Little Busters!) [Chinese] [寂月汉化组]

[Yanagawa Rio] Doutei Lovers | Virgin Lovers - Ch 1- 10 [English] {Taihen Zombii, JT Anonymus}

(Reitaisai 13) [Little☆Hamlet (Kiira~)] Komeiji Satori no Nichijou (Touhou Project) [Chinese] [灵梦书院汉化]

(C54) [Cu-little2 (Betty & MAGI)] Cu-Little Okirunya　(Cowboy Bebop)

(C84) [Hamanasu Chaya (Hamanasu)] B=Witch! (Little Witch Academia) [Chinese] [师兄汉化]

[Porori] My First Time is with.... My Little Sister?! (Ongoing)

The cute little sister gets mindbroken by the savage and gross elder brother's evil hands!

[Yanagawa Rio] Otouto de Asobou | Playing With My Little Brother (Toshishita no Otokonoko.) [English]

[Z-Ton] Sue Inbreed (Bessatsu COMIC Anthurium Ningen Igaija Damedesuka?) [English] [4dawgz + Thetsuuyaku] [Colorized] [Decensored] [Digital]

[Fuyuno Mikan] Chiisai Onnanoko ga Suki de Nani ga Warui! | What's Wrong with Liking Little Girls!? [English] [Rin]

(C58) [Cu-little2 (Beti, Magi)] Cu-Little Wannyannya~ (Breath of Fire IV)

[Ohnuma Hiroshi] Chibikko-tachi wa Natsu no Nioi | Little Children Smell of Summer Ch. 1-5 [English] {Mistvern}

[Mimoton] Natsu Basu (Little Busters)

Shitagi Model no Doutei Chinpo Kucchaimashita! | I Sucked Off an Underwear Model's Virgin Dick!

(C51) [Cu-little2 (Beti, MAGI)] Cu-Little!! (Various)

(C73) [ARESTICA (Ariko Youichi)] Cherryade. (Little Busters!)

[Kimura Neito] Watashi wa Kore de Kazoku to Nakayoku Narimashita. Zenpen (Love Juice Fountain) [English] [Team Koinaka]

[Porori] My First Time is with.... My Little Sister?! Ch.18

[PASTEL WING (Kisaragi-MIC)] Costume ALaMode ～Marmalade Kiss～ (Little Busters!) [Digital]

[Tsutsumi Akari] Virgin White [English] =Team Vanilla= [Decensored]

[ro] Ookami to Akazukin Ch. 4 [English] [Decensored] [Digital]

(COMIC1☆4) [Casmania (Mozuya Murasaki)] Dameda Ore mou Imouto Shika Mienai...! | Rotten me! I have eyes only for my little sister...! (Dragon Quest VI) [English] =LWB=

(Reitaisai 13) [Little☆Hamlet (Kiira~)] Komeiji Satori no Nichijou (Touhou Project)

(C84) [23.4Do (Ichiri)] Illya ga Imouto nara Shikatanai! | I Can't Help It If Illya Is My Little Sister! (Fate/kaleid liner Prisma Illya) [English] [Doki Fansubs]

[Toruneko] Souda Imouto to Renshuu Shiyou. | I Know, I'll Practice With my Little Sister. (Anoko to Iikoto) [English] {5 a.m.}

[Mizone] Imouto to Hajimeru Kuromajutsu Gishiki | My Little Sister and her First Black Magic Ritual (COMIC Anthology QooPA Vol. 07) [English] [PSYN] [Digital]

(C76) [Mimoton] Naoe de Asobo (Little Busters!)

[Atori K] Imouto Bloomer | Little Sister Bloomers Ch. 2 [English] [Yoshio]

Return World 2

[Nishi Shizumu] Ane Komori | Shut-In Sister (COMIC Shingeki 2013-02) [English] [Kameden]

Pink ni Pop, Purin ni Pie (My Little Pony: Friendship Is Magic

(C82) [Alice no Takarabako (Mizuryu Kei)] MC Gakuen [English] {Chocolate Scans, Doujin-moe, Little White Butterflies}

[Happouvijin (yumoteliuce)] Tasting (Little Witch Academia) [Digital]

(C96) [Funiai-ice (Funiai Riko)] Aya-chan no Otouto-kun to Runrunrun | Doing Boppin' Things With Aya-chan's Little Brother (BanG Dream!) (English)

(C88) [Mekabu Fighter (Uirou)] Mekabu Witch Academia (Little Witch Academia)

[Little mantis (Kuloamaki)] Seku Pure!!! ~Sexual Predators~ [Chinese] [臭鼬娘漢化組]

Ane's Target - Sisters' Target

[Popochichi (Yahiro Pochi)] Imouto to Uchi Kiss | Kissing in the House with Little Sister [English] [Col. Sanders] [Digital]

(C92) [TIES (Takei Ooki)] COCOLOGIC [Chinese] [无毒汉化组]

(C89) [65535th Avenue. (Akahito)] Makoto Shower (Tokyo 7th Sisters)

[Chan Shiden] Can't My Little Sister Be My Girlfriend? (COMIC LO 2019-01) [English] [Digital] [Learn JP with H]

[Porori] My First Time is with.... My Little Sister?! Ch.10

[Story Circle (Tonari)] Downer-kei Gamer Otouto ga Nii-san Sukisuki Imouto Succubus ni Naru made | From a Downer Gamer Little Brother♂ to a Little Sister♀ Succubus Who Loves Nii-san [English] [Panatical] [Digital]

[Naokame] S&M ~Okuchi de Tokete Asoko de mo Tokeru~ | S&M ~Melts in Your Mouth and Between Your Legs~ (COMIC L.Q.M ~Little Queen Mount~ Vol. 1) [English] [MintVoid] [Decensored]

[Araki Akira] Imouto Game | Little Sister Game [English]

(C92) [Little Hamlet (Kiira)] Komeiji Satori no Aozora no Shita de.... (Touhou Project)

Warui Imouto ni wa Oshioki o | My Little Sister Needs to be Punished!

[PASTEL WING (Kisaragi-MIC)] Costume ALaMode ～Marmalade Kiss～ (Little Busters!)

(C88) [Little★Cosmos (Ayano Rumi)] Netorare Oujo Lucina (Fire Emblem: Awakening) [Sample]

I’m not a virgin

[Anthology] LQ -Little Queen- Vol. 17 [Digital]

(C83) [SENYEN (Yoshida Nami)] Oshikake!! Kudryavka (Little Busters!)

[Maeshima Ryou] Virgin Bitch (COMIC LO 2013-05) [English] [Facedesk]

[Cu-little2 (Betty, MAGI)] Cu-Little Mint-chan Nya~ (DEWPRISM / Threads Of Fate)

[Aa Doushiyou (Pi-ko)] Ore no Imouto Tachi ga Hatsujouki na Wakega nai | There's No Way My Little Sister And Her Friend Are In Heat! (Ore no Imouto ga Konna ni Kawaii Wake ga Nai) [English] [Zettai Ryouiki] [Digital]

[Magukappu (Magukappu)] Dai Akko (Little Witch Academia)

[Shinobu Tanei] Imouto Netsuai Ryouiki | Little Stepsister Love Space Ch. 1-3 [English] {Tadanohito}

[TSF no F (Taniguchi-san)] Imouto Install | Little Sister Installation [English]

(C92) [Shoujo Kishidan (Oyari Ashito)] Mitsugetsu no Osanazuma - Honeymoon with little wives (Eromanga Sensei) [Chinese] [無邪気漢化組]

Little Red [English] [Rewrite] [Goat]

[Kouzuki Rio] Imouto Saimin Choukyou Manual | Little Sister Hypnosis Training Manual Ch. 1 [English] {desudesu}

Kosu Imo | Cosplay Little Sister

(COMIC1☆5) [Number2 (Takuji)] Ore no Imouto to, Imouto no Tomodachi ni Motto Nama de Nakadashi suru. | I'll Cum Inside My Little Sister, and Her Friends Too (Ore no Imouto ga Konna ni Kawaii Wake ga Nai) [English] [Belldandy100] [Decensored]

(C83) [Tennenseki (Suzuri)] Ritobasu Soushuuhen (Little Busters!)

[Porori] My First Time is with.... My Little Sister?! Ch.13

Hito wa Mikake ni | Judge a person by their looks

(C83) [Kouzaka-san to Makino Jimusho (Kouzaka Kouhei, Taki Minashika)] SISTERS -Natsu no Saigo no Hi- ART WORK (SISTERS -Natsu no Saigo no Hi-)

(C74) [Sakuranbo Little (Kanbayashi Chiko)] Otonya no Jikan (Kodomo no Jikan)

(C82) [Takanaedoko (Takanae Kyourin)] Imouto no Otetsudai 3 | Little Sister Helper 3 [English] (Hennojin)

Yariman ni Koise Shi Okasare Doutei | Virgin In Love Violated By a Slut

[Juan Gotoh] Doutei Senka | Virgin Special Course [English] {SaHa}

[Little mantis (Kuloamaki)] PEKEPEKE [Chinese] [臭鼬娘漢化組]

[Porori] My First Time is with.... My Little Sister?! (Ongoing)

(C54) [Cu-little2 (Betty & MAGI)] Cu-Little Okirunya (Cowboy Bebop) [English]

[Kokonoki Nao] Virgin [English] [tadanohito]

(C94) [Little Hamlet (Kiira)] Hatoba Tsugu no Kono Ko ni Shimashita | Hatoba Tsugu Became My Daughter (Hatoba Tsugu) [English] [DKKMD Translations]

(C59) [Little Kinetic (Morino Usagi)] Chokotone (Chobits)

[Fukumaaya] Uiuishii Imouto | Naive Little Sister (Monthly QooPA 2014-08) [English] [Crystalium]

[Anthology] LQ -Little Queen- Vol. 4 [Digital]

(CR33) [Ikaruganomiya (Umayadono Ohji)] Kinshin Soukan Kinki 6 (Sister Princess)

(CR34) [Virgin Virus (Matsumi Jun)] EPISODE LADYMAID ONE. ~BRAIN COPY~

(C92) [Little Hamlet (Kiira)] Komeiji Satori no Aozora no Shita de.... (Touhou Project)

A Little Complex [English] [Rewrite] [olddog51]

Little Ace Ch. 1, 2

[Calpis Koubou] How To Get More Intimate With Your Little Sister

(C80) [Little Hamlet, Nymphet (Ra Kii)] Kichiku Ou Reimu - Touhou Koumakyou Kouryakubon -Koumakan Hen- (Touhou Project) [English] [watisit]

Boku wa Imouto ni Katenai. 2 | I Can't Win Against My Little Sister. 2

Lust Alert If... ~Danger! Assault on a Virgin!~

[JACK-POT (Jyura)] RAIN -Kokuhaku shite Kita no wa Imouto datta- | RAIN -It Was My Sister Who Confessed to Me- [English] [Nisor] [Digital]

My First Time is with.... My Little Sister?! Ch.25

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-6 [English] [Tadanohito]

Imouto to Konma Ponchi | Konma Ponchi With My Little Sister

(C93) [TEBACO (tama)] Summer holiday, Again. (Little Witch Academia) [English] [/u/scanlations]

[Himeshibori (YAMATO)] Sasami ga Oshikko o Gaman Shite Omorashi Suru Hon (Little Busters!) [Digital]

(CT15) [LOST VERMILLION (Kamiya Chiaki)] Maple Syrup (Little Busters!)

[Pizanuko] Little Sister (COMIC LO 2019-02) [English] {Mistvern} [Digital]

Imouto wa Juken ni Ochita | My little sister failed her entrance exam.

Nashikuzushi Little by Little

Gimai Himari | My Stepsister Himari

Kirino Imouto Nama Chippai Nakadashi

[Miyano Kintarou] Hishojo List | Non-Virgin List 1-3 [English] {darknight} [Digital]

Doutei to Hisai | The Virgin Boy and the Secret Festival

[Mizu] Sharing the Blame ~In any case, I love my little sister~ [English] {5 a.m.}

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-3 [English] {Tadanohito}

(C74) [Kikakugai.Extend (itotin)] Inu Neko Busters (Little Busters!)

[Porori] My First Time is with.... My Little Sister?! (Ongoing)

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-2 [English] {Tadanohito}

[Kojima Miu] Ore no Kaa-san ga Itsu made mo Erosugiru Ch. 1-3 [English] [Shapes]

[Tsutsumi Akari] Virgin White [English] =Team Vanilla=

[Shinooka Homare] Anta wa Atashi no Ottoman | Little Brother, You Are My Ottoman (Girls forM Vol. 09) [English] {5 a.m.}

(C88) [Little Hamlet (Kiira)] Komeiji Satori no Elevator wa Toilet ja Arimasen (Touhou Project) [Chinese] [CE汉化组]

[KES3000 (Onaho Gonzaemon Full Bokkinosuke)] LITTLE WITCH SEX ACADEMIA (Little Witch Academia) [Digital]

(C60) [Yoru no Benkyoukai (Fumi Hiro)] Second Strike (Sister Princess)

[Juan Gotoh] Imouto wa Bitch | My Little Sister's A Bitch (COMIC Masyo 2009-08) [English] [SirC]

(CR32) [Studio BIG-X (Arino Hiroshi)] Mousou Mini Theater 9 (Sister Princess)

[Little mantis (Kuloamaki)] Sanistand [Digital]

[Minatoya Shunsaku] Himitsu no Roshutsu Kids | The Public Player, My Little Girl

(COMIC1☆5) [Number2 (Takuji)] Ore no Imouto to, Imouto no Tomodachi ni Motto Nama de Nakadashi suru. | I'll Cum Inside My Little Sister, and Her Friends Too (Ore no Imouto ga Konna ni Kawaii Wake ga Nai) [English] =LWB= [Decensored]

Ore no Imo Oto | My Little Sisbro

(C92) [DAICHIBOKUJOU (Makiba)] xxx (Little Witch Academia)

(Reitaisai 11) [Little Hamlet (Kiira)] Komeiji Satori A~shitai Ko~shitai (Touhou Project)

(C82) [Takanaedoko (Takanae Kyourin)] Imouto no Otetsudai 3 | Little Sister Helper 3 [English] {Hennojin}

(C91) [squeezecandyheaven (Ichihaya)] Imouto wa Sickness no Omake | My Little Sister is Sickly: Extra Story [English] [ATF]

[Morishima Kon] Virgin Sample (Oneshota Dish) [English] {Hennojin}

Omae Mada Imouto o Onaho ni Shite Nee no? | Are You Still Not Using Your Little Sister As An Onahole?

(C84) [Hamanasu Chaya (Hamanasu)] B=Witch! (Little Witch Academia) [English] [KirbyDances + deadFreak]

(C81) [kamisaka-san to makino jimusho] SISTERS ～ natsu no samenai hi ～ (SISTERS)

[Little Mermaid Henshuubu (Various)] Live! (Tenchi Muyo!)

[Ponkotsu System (Mitei)] Oppai no Ookina Imouto wa Suki Desuka? | Do You Like a Little Sister with Big Boobs? [English] [Degenerate_X]

(C89) [Cambropachycope (Soso-Zagri)] Onee-chan ni Onedari!

[Agata] ChinTrai Quest -Shota Yuusha no Haramase Chin Douchuu- | Dick Training Quest ~Little Hero's Conception Journey~ (Ane x Pako²) [English] =TLL + CW=

Childhood Destruction ~ Big Red Riding Hood and The little Wolf

Doutei Soukan l Virgin Adultery

[Little mantis (Kuloamaki)] Seku Pure!!!2 ~Sexual Predators~ [Chinese] [臭鼬娘漢化組]

[Calpis Koubou] Nakayohi Kyoudai - Imouto to Nakayoku Dekiru Ikutsuka no Houhou | How To Get More Intimate With Your Little Sister [English] =LWB=

(C78) [Little★Cosmos (Ayano Rumi)] Hazunde Catch! (HeartCatch PreCure!)

[Yukiji Shia] Minamino-san Ka no Bijin Shimai!? | The Beautiful Sisters of the Minamino Household!? (Onedari Seeshi) [English] {Hennojin}

(C86) [Little Hamlet (Kiira)] Komeiji Satori to Aru Natsu no Hi... (Touhou Project)

[Porori] My First Time is with.... My Little Sister?! Ch.26

[Little mantis (Kuloamaki)] Shota Kan [Digital]

[Tiger] Yuuwaku・Imouto #1 Onii-chan chi ni Otomari | Little Sister Temptation #1 Staying at Onii-chan's apartment (COMIC Reboot Vol. 06) [English] [Digital]

My Little Princess

[Mikekodou (Mikeko)] Imouto Zirusi (Sister Princess)

(C37) [Little Mermaid Henshuubu (Various)] LITTL MREMAID SELLECT (Urusei Yatsura, Maison Ikkoku)