Virgin Sis

[Kokonoki Nao] Virgin [Digital]

Madoka Oneechan to Chiisai Homura Chan

(COMIC1☆14) [squeezecandyheaven (Ichihaya)] Imouto wa Genius + Omake | My Little Sister Is a Genius + Bonus Story [English] [sneikkimies]

Sisters Cock [English] [Rewrite]

(Mimiket 5) [Imomuya Honpo (Azuma Yuki)] Oniisama e... 2.6 Mimiket 5 Kinen Copy Bon (Sister Princess)

[Miharu] Imouto no Betatsuku Hada to Sono Kusai | My Little Sister's Sticky Body and Odor (COMIC Masyo 2008-12) [English] [desudesu]

[Nipopo Crisis (Genka Ichien)] Gyaku Funsha Baby

Only for Sis [English] [Rewrite] [EZ Rewriter]

[Virgin Virus2 (Matsumi Jun)] O jou no Tsuyosugiru Seiyoku

[Yanagawa Rio] Doutei Lovers | Virgin Lovers - Ch 1- 10 [English] {Taihen Zombii, JT Anonymus}

Sis-pai Heaven - 01

[Tsubaki Jushirou] Daily Sisters [Chinese] [篆儀通文書坊漢化]

(Bokura no Grand Order) [Asanebou Crisis (Akaneman)] Shinshoku Seijo ChaosTide (Fate/Grand Order) [English] {darknight}

(C53) [Nipopo Crisis (Genka Ichien)] Ganso Ginga Hyouryuu Densetsu - Zouhokaiteiban (Galactic Drifter Vifam)

[Tomono Hiro] Cutie Beast (COMIC Penguin Club Sanzokuban 2015-05) [English] [Team Koinaka]

(C57) [Nipopo Crisis (Genka Ichien)] Microid M

[telomereNA (Gustav)] S-2:Scarlet Sisters (Touhou Project) [English] [desudesu] [Incomplete]

(C99) [Makino Jimusho (Taki Minashika)] SISTERS ~Kakusareta Kioku~ ACT.12 AKIKO Ⅵ + Omake (SISTERS -Natsu no Saigo no Hi-)

Taking Sis [English] [Rewrite] [olddog51]

(CR33) [Hakueki Shobou (A-Teru Haito)] Sisu Buri (Sister Princess)

(CrazyLyricBattle2) [Tore (KAZU)] YOKOHAMA 3P (Hypnosis Mic)

[Hiyori Hamster] Fukujuu no Noroi de Classmate o Onaho-ka Suru Hanashi | The Story of Turning Your Classmate into an Onahole through a Curse of Obedience [English] [Digital]

Queen's Sisters

(C41) [Naniwaya (Various)] Knight Vol. 2 (Bubblegum Crisis) [English]

Shitagi Model no Doutei Chinpo Kucchaimashita! | I Sucked Off an Underwear Model's Virgin Dick!

(C68) [Nipopo Crisis (Genka Ichien)] TORSO GIRLS (Various)

[Mikemono Yuu] Saimin Appli | Hypnosis App (COMIC Koh Vol. 7) [English] [N04h]

[Ashiomi Masato] Everyday Sisters [Chinese] [瓜皮汉化]

[Juan Gotoh] Nee-chan no Oshigoto wa... | My Older Sister's Job... (COMIC Masyo 2013-02) [English] [Busy Bee Translations] [Decensored]

Big Breasts Female Boss Hypnosis

[ban] Crisis Teacher(COMIC-X-EROS #24)[Chinese]

[Tsutsumi Akari] Virgin White [English] =Team Vanilla= [Decensored]

(C92) [Asanebou Crisis (Akaneman)] Gomennasai Senpai (Fate/Grand Order)

[Michiking] Ane Taiken Shuukan | The Older Sister Experience for a Week [English] [PSYN] [Digital]

Neme-sama no Geboku Choukyou

Sisters Sandwich

[Fumihiko] Sis Bro (COMIC Megastore 2012-08)

[Toruneko] Souda Imouto to Renshuu Shiyou. | I Know, I'll Practice With my Little Sister. (Anoko to Iikoto) [English] {5 a.m.}

[Ashiomi Masato] Everyday Sisters

[Touma Itsuki] Ane-sama Inkou - Sister's Sexy Smell [English] {desudesu}

[Yanagawa Rio] Tsundere Musume VS Doutei M Shounen | Awkward Girl VS Virginal Masochist Boy (Doutei Ouji) [English] [SubDESU-H]

[Granada Sky (Mogiki Hayami)] Milk Sister II [English] [Futanarifrank] [Digital]

[Nishi Shizumu] Ane Komori | Shut-In Sister (COMIC Shingeki 2013-02) [English] [Kameden]

[Honda Arima] The Slave of Three Sisters Ch.01-02

[ReDrop] Rumble Sisters (COMIC Megastore 2008-09) [English] [Desudesu]

[Makino Jimusho (Taki Minashika)] SISTERS ~Kakusareta Kioku~ ACT.11 AKIKO V (SISTERS -Natsu no Saigo no Hi-)

Knocking Up Sis [English] [Rewrite] [olddog51]

(C85) [Nipopo Crisis (Genka Ichien)] Tanoshii Koto o Naisho ni Shiyou. Yousumi Ban (Various)

[Jyaco] Zettai Kengen Sisters! | Absolute Authority Sisters [English] {doujin-moe.us + YQII}

Troilism

[Ayano Rena] Siscon - Sister Complex

Hearty Hybrid Household

[Kuroneko Smith] Oba-san no Karada ga Kimochi Yosugiru kara ~Boku no Oba-san wa Chou Meiki datta~ | My Aunt's Body is Irresistible. ~Her Hole is the Best~ (English) [Subsik]

Ane's Target - Sisters' Target

(C89) [65535th Avenue. (Akahito)] Makoto Shower (Tokyo 7th Sisters)

Trans Sisters

Tanimachi Maid - Love Love Sisters(english)

Fudeoro Sisters Ch.1-2

Imouto Tissue | Lil Sis' Tissues

Taking Advantage of Gray-chan Weakness, We Graduated from our Virginity.

(C95) [Kakukonoka (Menoko)] Onii-chan to Issho (Hypnosis Mic) [Chinese] [theoldestcat汉化]

[Tawara Hiryuu] Toshiue Zukushi Jukushita Sanshimai | The Three Older, Mature Sisters Next Door 1-2 [English] {darknight}

I’m not a virgin

(C69) [Nipopo Crisis (Genka Ichien)] Natsukorokei. (Various)

[Takuwan] Imouto wa Boku no Ayatsuri Figure | My Sister Is My Puppet (COMIC Shingeki 2016-03) [English]

[Bloody Okojo (Akutagawa Manbou, Takakujyu)] Brother and Sisters (Bakemonogatari) [English]

[Mikemono Yuu] Soku Hame! Onee-chans | Soku Hame! Sisters (COMIC Koh Vol. 5) [Chinese] [無邪気漢化組]

[Fumihiko] Sis Bro (COMIC Megastore 2012-08) [English] [Life4Kaoru]

Sis-pai Heaven!

[Maeshima Ryou] Virgin Bitch (COMIC LO 2013-05) [English] [Facedesk]

(COMIC1☆2) [Himawari-Noren (Chihiro Aoi)] Sai Nounin Ouenka (Kiss x Sis)

[Hen] Suto Sis | Stalking Sister (COMIC Tenma 2014-10) [English] [CGRascal]

(Futaket 10.5) [Doronuma Kyoudai (RED-RUM)] Futa Ona Tomoe VS Ootsubu Natto

[Hisasi] Sisters Ring (Futanarikko Fantasia) [English]

[Kouzuki Rio] Imouto Saimin Choukyou Manual | Little Sister Hypnosis Training Manual Ch. 1 [English] {desudesu}

(Crazy Lyric Battle 2) [Inukare (Inuyashiki)] Makenai Otoko (Hypnosis Mic)

[Shinama] Ana kara Imouto | With My Sister Through A Hole (COMIC Megastore 2011-09) [English] [4dawgz + Thetsuuyaku]

[Kokuriu] Castrato (Girls forM Vol. 04) [English] =RED=

(C83) [Kouzaka-san to Makino Jimusho (Kouzaka Kouhei, Taki Minashika)] SISTERS -Natsu no Saigo no Hi- ART WORK (SISTERS -Natsu no Saigo no Hi-)

(C64) [Nipopo Crisis (Genka Ichien)] Mokusakueki (Bishoujo Senshi Sailor Moon)

[Yukiyanagi] Shikatte! Futago Shimai | Scold Me! Twins Sisters (Complete) [ENG] [Yoroshii+SGC]

Watashi de Doutei Suteru Ki? | Am I Going to Lose My Virginity?

(C68) [Shimoyakedou (Ouma Tokiichi)] Sisters' Syrups (Fate/stay night)

Yariman ni Koise Shi Okasare Doutei | Virgin In Love Violated By a Slut

(C78) [STUDIO PAL (Nanno Koto)] Asuka no Shojo Teki Shougeki | Asuka's Virgin Shock (Neon Genesis Evangelion) [English] [HMedia]

[Juan Gotoh] Doutei Senka | Virgin Special Course [English] {SaHa}

(CR35) [Nipopo Crisis (Genka Ichien)] Ichien Satsu (Various)

(CR18) [Nipopo Crisis (Genka Ichien, Haineken, Serikawa Kazumi)] Kuroha Dynamic Junbigou 2! (Tonde Buurin)

[Yomotsuka Tsukasa] Sakura Crisis! (Junai Practice) [English] [Noraneko] [Digital]

(COMIC1☆6) [Renai Mangaka (Naruse Hirofumi)] Koi no Fire Sisters (Nisemonogatari)

[Taniguchi-san] -Tamashii- INSERT LEVEL 2: Shojo Dorobou | -Soul- INSERT LEVEL 2: Virginity Thief (COMIC Unreal 2015-06 Vol. 55) [English] [jabbany & DYNAM]

Boisshu no Ane ni Renshudai to Shite Shibora reta | This Tomboy Sister Squeezes Me In for a Practice Session

[Kisaragi Gunma] HINA Project (Giri Giri Sisters) [Chinese] [Decensored] [Colorized]

[Kokonoki Nao] Virgin [English] [tadanohito]

(C92) [Apsis (Sisochair)] Mahoutsukai wa Kanjitai (Touhou Project)

Do You Love Your Big Sister [ENG]

Chuu Shite! Vampire Girls -Sisters Party- | Kiss Me! Vampire Girls -Sisters Party-

(C60) [HarthNir (Misakura Nankotsu)] Binzume Sisters 1-B (Guilty Gear, Sister Princess)

[Kojima Saya] Devilish Sisters [English] [life4kaoru]

Onee-chan ni Makasete! | Leave it to Sis!

(CR33) [Ikaruganomiya (Umayadono Ohji)] Kinshin Soukan Kinki 6 (Sister Princess)

(CR34) [Virgin Virus (Matsumi Jun)] EPISODE LADYMAID ONE. ~BRAIN COPY~

(COMIC1☆4) [SLASH (Mitsurugi Aoi)] Riko-nee no xxx (Kiss x Sis)

Ochita Ane ni Shiborarete | Being Sucked Dry by My Corrupted Big Sis

[Shinozaki Rei] Boku to Imouto / Me and Lil Sis [English]

Ero Hitozuma ga Chuunen Doutei Futari o Fudeoroshi | Happy Cuckold Husband Series No. 01: Sexy Wife Breaks In Two Middle Aged Virgins

Lust Alert If... ~Danger! Assault on a Virgin!~

My Mom Used To Be A Slut, And Now She Wants My Virginity

[Shinonome Tarou] Swing Out Sisters [English] [Lint]

(C85) [Succuma-ya (Fukumaaya)] Houkago Tokubetsu Saimin Gakushuu | A Special Hypnosis Lesson after School [English] [biribiri]

[JACK-POT (Jyura)] RAIN -Kokuhaku shite Kita no wa Imouto datta- | RAIN -It Was My Sister Who Confessed to Me- [English] [Nisor] [Digital]

[jyaco] Zettai Kengen Sisters! (COMIC Megastore 2011-07) [English] [Sling]

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-6 [English] [Tadanohito]

[Yui Toshiki] My Sisters Ch.1-8 [English]

[7cm (Nase)] Ako-nee to Issen o Koeta Hi. (Kiss x Sis) [Chinese] [CE家族社] [Digital]

(SC47) [HANA TO RIBON (Puripuri Uemon)] The Onee-chans (Kiss x Sis)

Kuro Gal JK o Tasuketara Doutei o Ubawarete Kayoizuma ni Natta Hanashi | A Black Gal JK Took My Virginity and Became My Wife Who Lives Apart When I Helped Her Out

Gimai Himari | My Stepsister Himari

(COMIC1☆7) [40010 1-GO (40010 Shisakugata)] NEMESIS-NAMESIS (To Love-Ru Darkness)

[Miyano Kintarou] Hishojo List | Non-Virgin List 1-3 [English] {darknight} [Digital]

Sister Crisis 02

REDLEVEL5

Doutei to Hisai | The Virgin Boy and the Secret Festival

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-3 [English] {Tadanohito}

[Shinobu Tanei] Little Stepsister Fantasy Virgin Ch. 1-2 [English] {Tadanohito}

[Kiya Shii] Mind of Sisters Ch. 1-3 [English] [Yoroshii, Doujin-Moe]

[Tsutsumi Akari] Virgin White [English] =Team Vanilla=

(C88) [FREAKS (Mike)] Shiokara DRUG | Squid Sisters Drug (Splatoon) [English] {thetsuuyaku}

(C95) [Inukare (Inuyashiki)] Tarinai Otoko (Hypnosis Mic)

(C84) [Makino Jimusho (Taki Minashika)] SISTERS ~Kakusareta Kioku~ Soushuuhen 1 (SISTERS -Natsu no Saigo no Hi-)

(C60) [Yoru no Benkyoukai (Fumi Hiro)] Second Strike (Sister Princess)

[Juan Gotoh] Imouto wa Bitch | My Little Sister's A Bitch (COMIC Masyo 2009-08) [English] [SirC]

(CR32) [Studio BIG-X (Arino Hiroshi)] Mousou Mini Theater 9 (Sister Princess)

The persistent son [English] [Rewrite] [Buhalka]

Sister Anna no Kenshin

(C51) [Nipopo Crisis (Genka Ichien)] Karanba (Various)

(C87) [Sissch (Sissch)] Yamazaki Sousuke no Koufukuron (Free!)

(C92) [Oasis+ (Usashiro Mani)] Mezase SNS Bae Master!

(C73) [2Stroke (YTS Takana)] 2STROKE Mach (Neon Genesis Evangelion)

[hypnosis] Tonari no Uchi no Hito

(C78) [STUDIO PAL (Nanno Koto)] Asuka no Shojo Teki Shougeki - Asuka's Virgin Shock (Neon Genesis Evangelion)

(COMIC1☆4) [PASTEL WING] Suzi×kiss ~Otouto to Kodukuri shicha Damedesuka~ (Kiss x Sis)

[Supe (Nakani)] Onei-chan to Guchi o Kiite Ageru Otouto no Hanashi - Tales of Onei-chan Oto-to | Older Sister and Complaint Listening Younger Brother [English] [Decensored]

[doujinshi anthology] Himitsu no Houkago (Azumanga Daioh, Sister Princess, Evangelion)

(C60) [Shinohara Heavy Industry (Various)] Sis-Con 2 (Sister Princess)

(CR33) [HarthNir (Misakura Nankotsu)] Binzume Sisters Binzume Imototachi 4 (Sister Princess)

[Morishima Kon] Virgin Sample (Oneshota Dish) [English] {Hennojin}

Choroimin2 | Easy Hypnosis 2 {Hennojin}

[Tensei-kun] Kyodai - Ane to!! | Siblings - With Sister!!(COMIC Masyo 2012-01)[English][Amoskandy][Digital]

[ArcS (Sakura Yuu)] BUNNY SISTERS (Ore no Imouto ga Konna ni Kawaii Wake ga Nai) [Digital]